Inoculating Bacteria into a new jar

In a new bottle of Luria Broth, pipette 1 ml of your chosen bacteria and drop into the jar of Luria Broth.

To inoculate, place the luria broth bottle, now inoculated with bacteria into the shaking incubator.

Turn on the incubator switch on the left hand side. After it has loaded completely, press the knob until you can move screens to the “Programs” module

In the programs module, choose program #2 and press the button and choose “Run”, you should see a small man running on the bottom of the screen

The following are the steps for this program:

Hello Everyone! I'm posting my protocol for mounting and imaging worms.

Method for Mounting Worms and Imaging

Mounting

Start by leaving the agar in high heat to melt it, and switch to low heat once it is liquefies

Create the anesthetics mix by taking 2 ul of 0.2% tricane (TR), 4 ul of 0.02% tetramisole, and 94 ul of M9 buffer and put into a seperate eppendorf tube for a total of 100 ul of anesthetics mix. Use the electronic pipette and adjust the amount that you will be extracting.

Once the agar is melted, take a droplet of it using a glass pipette and drop it onto a new glass slide. Use the glass slide with taped edges to softly flatten the agar drop, allowing it to solidify and create a thin surface. Slowly slide off the taped glass slide and leave the agar sheet on the new glass…

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